Journal: Cell Death & Disease

Article Title: Adipocyte death triggers a pro-inflammatory response and induces metabolic activation of resident macrophages

doi: 10.1038/s41419-021-03872-9

Figure Lengend Snippet: A – C Expression of M1 markers CD11c, CD86, and CD9 and D , E M2 markers CD206 and CD301 by ATMs of induced CLS and interstitial macrophages. F , G Expression of CD64 (all macrophages) or TREM2. Targeted adipocytes are marked by an asterisk. M2 ATMs outside of CLS are highlighted by arrows. Scale bars = 50 µm.

Article Snippet: After fixation, the tissue was washed with PBS, blocked with staining buffer (3% bovine serum albumin (BSA) in PBS) for 1 h, and stained with pre-labeled antibodies in staining buffer (1:100 for antibodies from BioLegend, San Diego, USA: CD9 [Cat# 124810], CD36 [Cat# 102610], CD38 [Cat# 102716], CD11c [Cat# 117312], CD64 [Cat# 139332], CD86 [Cat# 105020], CD274 [Cat# 124312], F4/80 [Cat# 123122] and from R&DSystems, Minneapolis, USA: TREM2 [Cat# FAB17291A]; 1:200 for antibodies from AbD serotec, BioRad, Feldkirchen, Germany: CD301 [Cat# MCA2392A647] and CD206 [Cat# MCA2235A647T] at 4 °C overnight.

Techniques: Expressing

Journal: Cell Death & Disease

Article Title: Adipocyte death triggers a pro-inflammatory response and induces metabolic activation of resident macrophages

doi: 10.1038/s41419-021-03872-9

Figure Lengend Snippet: Whole mount antibody staining of CLS formed in vivo in lean mice under homeostatic conditions. A – C ATMs in in vivo formed CLS express pro-inflammatory markers CD11c, CD86, and CD9, while interstitial ATMs are negative (highlighted by arrows). D , E Interstitial ATMs express the anti-inflammatory markers CD206 and CD301 (highlighted by arrows), while ATMs in CLS are negative. F , G Expression of CD64 (all macrophages) or TREM2 (highlighted by arrows). Asterisks mark adipocytes inside CLS. Scale bars = 100 µm.

Article Snippet: After fixation, the tissue was washed with PBS, blocked with staining buffer (3% bovine serum albumin (BSA) in PBS) for 1 h, and stained with pre-labeled antibodies in staining buffer (1:100 for antibodies from BioLegend, San Diego, USA: CD9 [Cat# 124810], CD36 [Cat# 102610], CD38 [Cat# 102716], CD11c [Cat# 117312], CD64 [Cat# 139332], CD86 [Cat# 105020], CD274 [Cat# 124312], F4/80 [Cat# 123122] and from R&DSystems, Minneapolis, USA: TREM2 [Cat# FAB17291A]; 1:200 for antibodies from AbD serotec, BioRad, Feldkirchen, Germany: CD301 [Cat# MCA2392A647] and CD206 [Cat# MCA2235A647T] at 4 °C overnight.

Techniques: Staining, In Vivo, Expressing

Journal: Nature Communications

Article Title: Viral entry shapes HCMV latency establishment

doi: 10.1038/s41467-025-68063-y

Figure Lengend Snippet: a NRP2 expression in primary, THP-1 and Kasumi-3 monocytes and macrophages as measured by RNA-seq. Mono monocytes, Mac macrophages, RPM reads per million. n = 2. Source data are provided as a file. b Flow cytometry analysis of NRP2 versus IgG control cell surface staining in primary and THP1 monocytes and macrophages. c Flow cytometry analysis of THP1 macrophages with NRP2 or control CRISPR knockout, infected with HCMV-GFP. Analysis was performed at 3 dpi. d Flow cytometry analysis of THP1 macrophages, transfected with NRP2 and control siRNA 2 days before infection with HCMV-GFP. Analysis was performed at 3 dpi. Gating strategies for Fig. 6b–d are shown in Fig. .

Article Snippet: After blocking, cell staining was done using the following conjugated antibodies: allophycocyanin (APC)-conjugated mouse IgG2a anti-human neuropilin-2 (R&D systems, catalog no. FAB22151A) with allophycocyanin (APC)-conjugated mouse IgG2a control (R&D systems, catalog no. IC003A).

Techniques: Expressing, RNA Sequencing, Flow Cytometry, Control, Staining, CRISPR, Knock-Out, Infection, Transfection

Journal: iScience

Article Title: Human iPSC modeling recapitulates in vivo sympathoadrenal development and reveals an aberrant developmental subpopulation in familial neuroblastoma

doi: 10.1016/j.isci.2023.108096

Figure Lengend Snippet:

Article Snippet: The development of meso-endoderm was validated using immunofluorescence staining for Troponin T (abcam, Cat nr. ab228847) and Brachyury (R&D systems, Cat nr. IC2085A).

Techniques: Recombinant, Saline, Derivative Assay, Staining, Amplification, Sequencing, Software, In Vivo

Journal: Nature

Article Title: Stem cell function and stress response are controlled by protein synthesis

doi: 10.1038/nature18282

Figure Lengend Snippet: a , Hair cycle stages and genetic lineage marking using K19- and Lgr5 tdTomato (tdTom) mice. Cell surface markers to isolated bulge stem cells are CD34 and Itgα6. Telogen: stem cells (CD34 + /Itgα6 + ) are quiescent and resting in the bulge (BG). Early anagen: stem cells divide and give rise to committed progenitors in the hair germ (HG), which then grow downwards into the bulb (BU) surrounding the dermal papilla (DP). Late anagen: cells differentiate upwards to form the hair. Catagen: intermediate phase, when the hair bulb degenerates into a new resting bulge. IFE: interfollicular epidermis; SG: sebaceous glands. Mouse transgenes label K19 (red)- and Lgr5 (orange)-positive stem cells and their progeny. b , OP-puro detection in mouse epidermis at all hair cycle stages. Dotted lines: hair follicle and epidermal basal layer. Arrows: OP-puro high cells in the hair follicle. Arrowheads: OP-puro low cells in the interfollicular epidermis. Nuclei (DAPI). c , tdTomato and OP-puro detection in back skin of K19tdTom and Lgr5tdTom mice in telogen and late anagen. Arrows: Tomato + cells. Arrowheads: Tomato + /OP-puro high cells. Dotted line: lower bulge. Merged panels in . e , Hair follicle lineages and differentiation markers used in . ORS: outer root sheet; Cp: Companion layer; IRS: inner root sheet; He: Henle’s layer; Hu: Huxley layer; Ci: cuticle of inner root sheet; Ch: cuticle; Co: cortex; Me: Medulla. f , P-cadherin and OP-puro detection in a late anagen. Scale bars: 50 μm.

Article Snippet: Primary antibodies were used at the following dilutions: rabbit polyclonal to RFP (for tdTomato) (1:1000; Rockland inc., 600-401-379), mouse monoclonal to Dlx3 (1:200, Abnova, H00001747-A01), rabbit polyclonal to K6 (1:200, Abcam, ab24646), mouse monoclonal to Gata3 (1:50, Santa Cruz Biotech, sc-268), guinea pig polyclonal to K31 and K72 (1:200, Progen, GP-hHa1 and GP-K6irs2), rabbit polyclonal anti c-maf (1:100, Bethyl, A300-613A), mouse monoclonal to Lef1 (1:50, Santa Cruz Biotech, sc-81470), goat polyclonal to P-cadherin (1:100, R&D systems, FAB761A), rabbit monoclonal antibody to Ki67 (1:200; SP6, Vector Labs, VP-RM04), mouse monoclonal anti-mouse Keratin 15 (1:1000 ), β-Catenin (1:200, Santa Cruz Biotech, sc-7199), rat monoclonal anti-Itgβ1 (1:200, clone HMβ1-1, BioLegend, 102203), rabbit polyclonal anti mouse keratin 10 (1:500; Covance, PRB-159P), rabbit polyclonal anti-NSun2 (1:500; Aviva Systems Biology, ARP48811_P050), rabbit polyclonal anti-human NSun2 (MetA, 1:500 ), rat monoclonal anti-Itgα6 (1:500; GoH3, eBioscience, 14-0495), rat monoclonal anti-CD44 (1:200, IM7, BioLegend, 103004), anti-mouse Podoplanin (1:500, clone 8.1.1, eBioscience, 14-5381), rat monoclonal anti-BrdU (1:100; Abcam, ab6326), rabbit polyclonal anti-Laminin alpha 5 (1:100, Abcam, ab75344), mouse monoclonal anti-cytokeratin 8 (1:100, TROMA-I, DSHB, US), rabbit polyclonal to Slug (1:200, Cell Signaling, 9585P), chicken polyclonal anti-GFP (1:200, Abcam, ab13970), rabbit polyclonal anti-p53 (1:100, CM5, Novocastra, NCL-p53-CM5p), and rabbit anti human Involucrin (SY5 clone, 1:200, Abcam, ab80530).

Techniques: Isolation